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优化细胞分裂监测细胞示踪染料的染色和扩散建模方法

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

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优化细胞分裂监测细胞示踪染料的染色和扩散建模方法

DOI:

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22:49 min

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December 13, 2012

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Joseph D. Tario Jr., Kristen Humphrey, Andrew D. Bantly, Katharine A. Muirhead, Jonni S. Moore, Paul K. Wallace

¹Department of Flow and Image Cytometry,Roswell Park Cancer Institute, ²Flow Cytometry & Cell Sorting Resource Laboratory,University of Pennsylvania , ³SciGro, Inc., ⁴Department of Pathology and Laboratory Medicine,University of Pennsylvania

Capitoli

00:05Titolo
02:19General Membrane Labeling with PKH26 Cell Tracking Dye
05:43Flow Cytometer Setup and Staining Verification
07:34Proliferation Model Selection for Cell Division Monitoring by Dye Dilution
10:16Results: Optimization of Cell Tracking Dye Staining for Monitoring Cell Division
22:03Conclusion

Summary

Traduzione automatica

成功地利用监测免疫细胞的功能和增殖的细胞追踪染料涉及的几个关键步骤。我们描述的方法为：1）获得明亮，均匀，重现性好标签的膜染料; 2）选择荧光染料和数据采集条件; 3）选择一个模型来量化细胞增殖的基础上染料稀释。

Tags

Optimized Staining Proliferation Modeling Cell Division Monitoring Cell Tracking Dyes Flow Cytometry Image Cytometry Stem Cells Progenitor Cells Membrane Transfer Precursor Cell Proliferation Immune Regulatory Cells Effector Cell Function Commercially Available Dyes Membrane Dyes Protein Dyes Multicolor Studies Optimal Use Of Dyes

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