设计和组装的超轻型电动微型硬盘（Microdrive）慢性神经录音的小动物
Summary
一个超轻型电动微硬盘的设计，制造和装配。该设备提供了成本效益和易于使用的解决方案，单台小行为的动物慢性录音。
Abstract
的能力，长期记录神经元在自由活动的动物的种群已被证明是非常宝贵的工具，用于解剖的神经回路的功能相关的各种自然行为，包括导航1，决策2,3，和上一代的复杂的运动序列4 ，5,6。在精密加工方面的进展已可用于制造重量轻的设备，适合慢性录音的小动物，如小鼠和鸣禽。调整电极的位置与远程控制电机的能力进一步提高各种行为背景的记录产量的减少动物的处理6,7。
在这里，我们描述了一个协议，以建立一个超轻型电动微硬盘为长期的慢性记录的小动物。我们的设计是从早前公布的第7版，已被改编为易用性和成本有效的veness更加实用和广泛的研究人员访问。这种成熟的设计8,9,10,11允许的精细电极，远程定位〜5毫米的范围内，重量不超过750毫克时完全组装。我们提出如何构建和组装这些驱动器，包括三维CAD图纸的所有自定义的微硬盘组件的完整的协议。
Protocol
1。组件概述一个完整的微硬盘组成的几个主要部件（ 图1）：作为上层建筑具有精细螺纹输出轴的驱动器，电动机，带有电极和提供了一个电连接的点的螺纹班车的机箱，以及一个Omnetics（或同等学历）连接器。 底盘，电极班车，电极穿梭管的自定义组件的设计（SolidWorks的三维CAD软件）和加工的精密加工。其它所有组件都是市售的。 底盘和穿梭从轻量级的聚醚?…
Discussion
这里介绍的协议将导致一个能够以最小的运动伪影的高品质的录音设备，只有采取适当的照顾与建设。适合穿梭在机箱的如果至关重要太紧张，电机过载的风险是高过于宽松，显着的运动伪影的风险是高。一个理想的选择，可以让穿梭旅行的整个长度的螺纹轴的位置或抖动没有倾斜了。
记录电极的选择同样是重要的;材料，阻抗，绝缘，和小费档案中的选择可能会影响组织的?…
Divulgazioni
The authors have nothing to disclose.
Acknowledgements
这项工作是由酯和约瑟夫·麦克奈特Klingenstein基金，捐赠基金，NINDS 1R01NS066408-01A1。
Materials
| Name of item | Company | Catalogue number | Comments |
| Chassis | custom | Cut from PEI | |
| Electrode Shuttle | custom | Cut from PEI | |
| Shuttle Tubes | custom | Cut from Stainless Steel | |
| Connector | Omnetics | A7886-001 | Mates to A7877-001 |
| Motor w/ Gearhead | Faulhaber | 0206-A-001-B-021-47:1 | |
| Wire Guide | Small Parts, Inc | SWPT-0113-12 | |
| Electrode Guide | Small Parts, Inc | SWPT-0045-12 | |
| 10MΩ Pt-Ir electrodes | Microprobes, Inc | PI2PT310.0H3 | |
| Platinum Wire | A-M Systems | 772000 | For electrode wires |
| Silver Wire | A-M Systems | 786000 | For ground wire |
| Tungsten Wire | A-M Systems | 797000 | For electrode pins |
| Transparency | 3M | AF4300 | |
| Torr Seal | Varian | 9530001 | |
| Kwik-Cast | WPI | KWIK-CAST | |
| Cyanoacrylate | Krazy Glue | KG517 | |
| Fast-Set Epoxy | Hardman | 04001 | |
| Light Mineral Oil | Sigma | M5310 | |
| Chlorine bleach | |||
| Diagonal cutters | |||
| Scalpel blade | |||
| Forceps | |||
| Drive jig | custom | Epoxy the mating connector to a syringe or stick | |
| Small Vice |
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Tags
Ultra-light Motorized MicrodriveChronic Neural RecordingsSmall AnimalsPrecision MachiningElectrode Position AdjustmentRemotely Controlled MotorsRecording YieldBehavioral ContextsLong-term Chronic RecordingsCost-effectivenessPracticalityAccessibilityFine Positioning Of Electrodes3D CAD DrawingsCustom Microdrive Components
Citazione di questo articolo
Otchy, T. M., Ӧlveczky, B. P. Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals. J. Vis. Exp. (69), e4314, doi:10.3791/4314 (2012).