Journal
/
/
腸管上皮細胞の腸内細菌の侵入<em>インビトロ</em>劇的垂直拡散室モデルを用いた強化され
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
DOI:

10:52 min

October 22, 2013

, , ,

Chapters

  • 00:05Title
  • 01:47Growth of Intestinal Epithelial Cell (IEC) Monolayers on 0.4 µm Filters
  • 02:22Vertical Diffusion Chamber (VDC) Co-culture Assay Preparation
  • 04:21Setting up of the VDC
  • 06:14Disassembling of the VDC after Co-culture
  • 08:34Results: The VDC Model Facilitates Greater Numbers of Bacterial-IEC Interactions and Invasions than Standard Cell Culture Assay Conditions
  • 10:07Conclusion

Summary

Automatic Translation

好気的条件下で細菌の付着および浸潤を調べるための細胞培養アッセイを行うと、通常の典型的である<em生体内で></em>環境。垂直拡散室モデルは、ヒト病原体の相互作用の研究を可能にします<em>カンピロバクター</emもっと下の腸上皮細胞と><em生体内で></em条件>のような、強化された細菌の侵入になる。

Related Videos

Read Article