Materials
Name | Company | Catalog Number | Comments |
RNAqueous-Micro Kit | Life Technologies GmbH, Germany | AM1931 | Isolation of total RNA, 50 samples |
Dynabeads mRNA DIRECT Micro Kit | Life Technologies GmbH, Germany | 61011 | 5 ml Dynabeads Oligo (dT)25 |
DynaMag-2 magnet | Life Technologies GmbH, Germany | 12321D | |
SuperScript CellsDirect cDNA Synthesis System | Life Technologies GmbH, Germany | 18080-200 | 25 reactions |
RNaseOUT | Life Technologies GmbH, Germany | 10777-019 | Rnase inhibitor, 5,000 units |
Random Hexamers (0.4 μg/μl) | QIAGEN GmbH, Germany | 79236 | 100 μl |
10 mM dNTP Mix, molecular biology grade | Fermentas GmbH, Germany | R0192 | 1 ml |
Taq DNA polymerase, (recombinant) | Fermentas GmbH, Germany | EP0402 | Supplied with 10x Taq buffer (with KCl or (NH4)2SO4) and 25 mM MgCl2 |
TURBO DNase | Life Technologies GmbH, Germany | AM2238 | 1,000 units |
Agarose | Carl Roth GmbH Co. Germany | T846.3 | |
GeneRuler Low Range DNA ladder, ready-to-use | Fermentas GmbH, Germany | SM1193 | 50 μg |
Brilliant II SYBR Green QPCR Master Mix | Agilent Technologies, Inc., USA | 600828 | Single kit |
RNaseZAP | Sigma-Aldrich, Taufkirchen, Germany | R2020 | |
Razor blade | Carl Roth GmbH Co., Germany | CK08.1 | |
Tweezers | Carl Roth GmbH Co., Germany | 2801.1 / 2855.1 | |
GE NanoVue spectrophotometer | GE Healthcare Europe GmbH, Germany | 28-9569-62 | |
Mastercycler gradient | Eppendorf AG, Hamburg, Germany | 5331 000.010 | |
Mx3000P Real-Time PCR System | Stratagene, USA | 401403 | |
Agagel Standard Horizontal Gel Electrophoresis Chamber | Biometra | Discontinued | |
Micromanipulator | aura optik gmbh | aureka | Equipped with a microforceps |
Stereomicroscope | Carl Zeiss Microscopy, Jena, Germany | SteREO Lumar.V12 | Equipped with an AxioCam |
Lumar Filter set 01 | Carl Zeiss, Microscopy, Jena, Germany | 485001-0000-000 | UV filter |
Lumar Filter set 09 | Carl Zeiss, Microscopy, Jena, Germany | 485009-0000-000 | non-UV filter |
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