등쪽 뿌리 신경절 모델 : 암성 신경 침략의 생체 외 모델링에서
Summary
This video article shows the use of the dorsal root ganglia (DRG)/cancer cell model in pancreatic ductal adenocarcinoma.
Abstract
One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These neurotropic cancer cells have a unique ability to migrate unidirectionally along nerves towards the central nervous system (CNS). The dorsal root ganglia (DRG)/cancer cell model is a three dimensional (3D) in vitro model frequently used for studying the interaction between neural stroma and cancer cells. In this model, mouse or human cancer cell lines are grown in ECM adjacent to preparations of freshly dissociated cultured DRG. In this article, the DRG isolation protocol from mice, and implantation in petri dishes for co-culturing with pancreatic cancer cells are demonstrated. Five days after implantation, the cancer cells made contact with the DRG neurites. Later, these cells formed bridgeheads to facilitate more extensive polarized, neurotropic migration of cancer cells.
Introduction
직접 침윤, 림프 확산 및 hematogenic 확산 : 고체 종양은 세 가지 방법으로 전파. 그러나, 자주 신경을 따라, 보급을 무시 암 확산의 제 4 수단이있다. 암성 신경 침윤 (CNI)는 특히 두경부 1 전립선 2, 3 및 췌장 암의 암 확산의 공지의 경로이다. 4-8 CNI 선도 췌장 선암 개인의 80 % 이상에서 발생 복강 신경절 신경을 통해 확산 복막 종양. 이러한 neurotropic 암 세포는 중추 신경계 (CNS) 방향으로 신경을 따라 일방향으로 이주 할 수있는 독특한 능력을 가지고있다. (9)이 결과는 신경초 미세이 악성 성장을 지원하는 인자를 제공하는, 암 세포에 의해 이용 될 수 있다는 것을 시사한다.
CNI 연구를위한 몇 체외 모델 중 하나는 후근 신경절 (DRG) / 암 세포 모델입니다. 이 모드엘 자주 갓 해리 배양 DRG의 준비에 인접한 세포 외 기질 (ECM)에서 재배되는이 모델, 마우스 또는 인간의 암 세포 라인에 신경 기질 암 세포 사이. 10-18 분비의 상호 작용을 연구하는 데 사용됩니다.
이 비디오 기사는 췌장 도관 선암에서 체외 CNI의 응용 프로그램을 보여줍니다.
Protocol
Representative Results
Discussion
이 문서에서는 신경 틈새에서 암의 미세 환경의 DRG 모델을 되풀이되었습니다 체외 모델을 제시한다. 비디오는 같은 마우스에서 DRG, 그 추출 및 ECM의 마지막으로, 그 배양으로 해부학 적 랜드 마크를 인식에서 시작하여 모든 단계를 보여줍니다. 공동 배양 암세포 나란히 DRG 또한 제공된다. 체외에서 신경초 틈새 미세 환경 연구를위한이 모델은 필수적 만드는 문헌에 기재된 체외</e…
Divulgazioni
The authors have nothing to disclose.
Acknowledgements
Edith Suss-Toby is thanked for her assistance in the time-lapse microscopy and image analysis. Nofar Rada is thanked for the artistic work.
Materials
| Equipments: | |||
| Operating microscope | Leica | M205 | |
| Tiime Lapse System | Zeiss | ||
| Forceps | Sigma-Aldrich | F4142 | |
| Surgical blade | Sigma-Aldrich | Z309036 | |
| Scissors | Sigma-Aldrich | S3271 | |
| 35mm petri dishes, glass bottom | de groot | 60-627860 | |
| Name | Company | Catalog Number | Comments |
| Materials: | |||
| 70% ethanol | sigma | ||
| Cold PBS | Biological industries | 02-023-1A | |
| DMEM | Biological industries | 01-055-1A | |
| FCS | Rhenium | 10108165 | |
| Penicillin and streptomycin | Biological industries | 01-031-1B | |
| Sodium Pyruvate | Biological industries | 03-042-1B | |
| L-Glutamine | Biological industries | 03-020-1B | |
| Growth factor depleted matrigel | Trevigen | 3433-005-01 |
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