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自发性乳腺癌转移的原位小鼠模型

JoVE Journal
Medicina

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JoVE Journal Medicina

An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

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自发性乳腺癌转移的原位小鼠模型

DOI:

10.3791/54040-v

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08:11 min

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August 14, 2016

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Amy V. Paschall^2,3, Kebin Liu^2,3

¹Department of Biochemistry and Molecular Biology, Medical College of Georgia,Augusta University, ²Georgia Cancer Center,Augusta University, ³Charlie Norwood VA Medical Center

Capitoli

00:05Titolo
00:48Orthotopic Breast Cancer Tumor Establishment
02:17Live Mouse Bioluminescent Tumor Growth Imaging
03:24Surgical Primary Tumor Removal
04:36Lung Metastases Validation
06:13Results: Representative Evaluation of Spontaneous Breast Cancer Metastasis
07:00Conclusion

Summary

Traduzione automatica

原位乳腺癌原发肿瘤模型和手术切除原发肿瘤，延长生命鼠标产生自发转移的描述。肿瘤的生长和发展进行监测和通过萤光素酶荧光成像定量。

Tags

Orthotopic Mouse Model Spontaneous Breast Cancer Metastasis 4T1 Luciferase Cells BALB/c Mice Bioluminescence Imaging Primary Tumor Growth Metastasis Kinetics

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