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皮肤去神经外科手术:一种用于皮肤病的小鼠模型测试要求的神经方法
JoVE Journal
Medicina
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JoVE Journal Medicina
Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease
DOI:

08:01 min

June 26, 2016

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Capitoli

  • 00:05Titolo
  • 00:43Collecting Skin Biopsies
  • 02:49Surgical Denervation
  • 05:36Behavioral Test of Denervation
  • 06:22Results: Touch Dome Cells and Merkel Cells Require Innervation
  • 07:20Conclusion

Summary

Traduzione automatica

This article includes detailed protocols for genetic labeling of mouse skin, surgical denervation, skin biopsy and visualizing labeled epithelia by whole-mount β-galactosidase staining. These methods can be used to test the requirement for nerves in mouse models of normal and pathological skin.

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