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G2-seq: 一种基于高通量测序技术的基因组晚期复制区域识别方法

JoVE Journal
Genetica

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JoVE Journal Genetica

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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G2-seq: 一种基于高通量测序技术的基因组晚期复制区域识别方法

DOI:

10.3791/56286-v

•

06:40 min

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March 22, 2018

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Eric J. Foss, Uyen Lao, Antonio Bedalov²

¹Division of Clinical Research,Fred Hutchinson Cancer Research Center, ²Departments of Medicine and Biochemistry,University of Washington

Capitoli

00:04Titolo
00:44Preparing Cells and Sorting Using Flow Cytometry
03:13DNA Extraction and Sequencing Library Preparation
05:20Results: Late Replicating Sites in Budding Yeast
06:14Conclusion

Summary

Traduzione automatica

我们描述了一种将流式细胞术和高通量测序相结合的技术来识别基因组的晚期复制区域。

Tags

G2-seq High-throughput Sequencing DNA Replication Cell Cycle Late Replicating Regions Genome Yeast Flow Cytometry Cell Sorting DNA Content G1 S G2 Phases

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