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Laser-Capture Microdissection RNA-sekventering for rumlige og tidsmæssige vævsspecifikke genekspressionsanalyser i planter
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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-sekventering for rumlige og tidsmæssige vævsspecifikke genekspressionsanalyser i planter

DOI:
10.3791/61517-v

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08:33 min

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August 05, 2020

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, , , , ,
1Department of Animal, Plant and Soil Science, AgriBio Building,La Trobe University, 2Australian Research Council Centre of Excellence in Plant Energy Biology,La Trobe University, 3Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building,La Trobe University

Chapters

  • 00:04Introduction
  • 01:11Tissue Fixation
  • 01:47Tissue Processing
  • 02:38Paraffin Embedding
  • 03:21Polyethylene Naphthalate (PEN) Membrane Slide Preparation
  • 03:49Sectioning
  • 04:53Laser-Capture Microdissection (LCM)
  • 06:14Results: Representative LCM Sample Acquisition and RNA Quantification
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

Præsenteret her er en protokol for laser-capture microdissection (LCM) af plantevæv. LCM er en mikroskopisk teknik til isolering af vævsområder på en forureningsfri måde. Proceduren omfatter vævsfiksering, paraffinindlejring, sektionsindtrækning, LCM- og RNA-ekstraktion. RNA anvendes i den downstream-vævsspecifikke, tidsmæssigt løste analyse af transskriptomer.

Tags

Laser-capture MicrodissectionRNA-sequencingSpatial TranscriptomeTemporal TranscriptomePlant CellsParaffin EmbeddingBarley SeedVacuum InfiltrationDehydrationXyleneParaffin Infiltration

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