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牛卵巢皮质组织培养
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Biologia
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JoVE Journal Biologia
Bovine Ovarian Cortex Tissue Culture
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牛卵巢皮质组织培养

DOI:
10.3791/61668-v

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06:32 min

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January 14, 2021

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, , ,
1Department of Animal Science,University of Nebraska-Lincoln, 2Department of Animal Production, School of Agriculture,University of Jordan

Capitoli

  • 00:05Introduction
  • 01:07Ovarian Cortical Culture Protocol
  • 03:15Media Collection, Imaging, and Downstream Processing
  • 04:39Results: Analysis of H & E Staining, Number of Follicles at Different Stages, and Collagen Staining of the Ovarian Cortex
  • 05:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文介绍了牛卵巢皮层的体外培养以及营养阶梯式饮食对卵巢微环境的影响。将卵巢皮层切片培养七天，并评估类固醇，细胞因子和卵泡分期。阶梯式饮食治疗增加了类固醇生成，导致培养物中的卵泡进展。

Tags

Bovine Ovarian CortexFollicle GrowthSteroidogenesisImmune MoleculesReproductive DisordersPolycystic Ovarian SyndromeOvarian MicroenvironmentIn Vitro Examination

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