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晚期胚胎 果蝇 性腺的解剖和活体成像
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
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晚期胚胎 果蝇 性腺的解剖和活体成像

DOI:
10.3791/61872-v

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09:08 min

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October 17, 2020

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, , ,
1Cell and Developmental Biology Department and the Penn Institute for Regenerative Medicine, Perelman School of Medicine,University of Pennsylvania

Capitoli

  • 00:05Introduction
  • 00:46Day-of-Dissection Preparation
  • 02:35Dissection
  • 04:53Imaging
  • 06:46Results: The Live-Imaging Results of the Cultured Gonads, Their Health, and Niche Compaction
  • 08:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提供了对晚期胚胎 果蝇 雄性性腺进行现场成像所需的解剖方案。该协议将允许在正常条件下或在转基因或药理学操作之后观察动态细胞过程。

Tags

Live ImagingGonadogenesisDissectionDrosophila EmbryoVitelline MembraneRinger’s SolutionHeptane GlueTungsten NeedlePoly-D-LysineCover Slip

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