腹部小鼠心脏移植模型中的血液回路重建
1Department of Pediatric and Adolescent Medicine,University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery,Chengdu Women’s and Children’s Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue,The first affiliated Hospital of Hainan Medical University, 4Department of Infectious Diseases, West German Centre of Infectious Diseases,Universitätsmedizin Essen, University Duisburg-Essen, 5Department of Nephrology,Hannover Medical School
Summary
演示了异位小鼠心脏移植模型中血液回路重建的新技术。
Abstract
小鼠异位性腹部心脏移植手术技术是移植免疫学研究的标准模型。在这里，介绍了异位性腹部心脏移植模型中经过修饰的血液回路重建的既定技术。这种方法使用胸内低静脉卡瓦（IIVC），而不是供体心脏的肺动脉，用于接受者低等静脉卡瓦的解剖。它促进和提高小鼠腹部心脏移植的成功率。
Introduction
小鼠异位性腹部心脏移植手术技术是移植免疫学1、2、3研究的标准模型。然而，这是非常具有挑战性的执行，这涉及到限制广泛使用这种模型4，5。
在传统的小鼠心脏移植（THTx）中，供体主动脉和接受者腹部主动脉为肛门，而肺动脉为接受者低维纳卡瓦6、7、8。
在这种修改后小鼠心脏移植技术中，供体主动脉与接受者腹部主动脉分离，供体IIVC与接受者低劣的静脉卡瓦（3、4、6）（图2和图3）有关。
Protocol
所有动物实验都是按照欧洲议会2010/63/欧盟关于保护用于科学目的的动物的指令（伦理委员会批准，#G1071/09）的指导方针进行的。 注：初步准备、麻醉、术后护理和监测工作与传统手术方法1、2、4相同。BALB/c 小鼠充当心脏捐献者，C57BL/6J 担任移植接受者。小鼠的年龄为8-12周，移植时体重约30克，在标准条?…
Representative Results
在这里，介绍了一种经过改进的小鼠异位腹部心脏移植技术，该技术以前在我们的实验室中已经开发出来，并且在过去16年中被证明是有用的。此前，据报道，在总共40例静脉卡瓦到静脉卡瓦（V-V组）相比，40例传统肺动脉静脉卡瓦（P-V组）解剖程序4（表1），船舶解剖病在V-V组服用20.8±1.3分钟， 明显低于P-V组（27.5±1.3分钟，第0.01页<）。暖缺血时间、总接受者…
Discussion
小鼠异位心脏移植手术技术极具挑战性，这涉及到这种模式的广泛应用受到限制。
传统技术的缺点之一是捐赠者肺动脉（PA）的长度有限。它通常长度约为2毫米，而我们模型中使用的供体心脏IIVC的长度一般约为1厘米（图2）。这意味着，在修改后的模型中，IIVC肛门切除术提供了更清晰的视线，使肛门手术得到改善，并防止过于严格的缝合线，甚至损害?…
Divulgazioni
The authors have nothing to disclose.
Acknowledgements
我们感谢徐云博士作为配音演员彭建华博士对视频编辑的帮助，感谢安妮卡·库克哈恩博士对她的评价和支持。这项工作部分得到了德国研究基金会（DFG）的支持，以促进国际合作（HO2581/4-1对AH）和中国国家科学基金会（NSFC;#81760291到FJ）。
Materials
| 30G-needles | Braun | 456300 | |
| acepromazine | CP Pharma | Tranquisol P | |
| BALB/c AnNCrl mice | Charles River. Germany | no catalog number | |
| Bepanthen eye ointment | Haus-Apotheke | PZN 01578675 | |
| Bonn Micro Forceps | FST | 11083-07 | |
| Box for insulation and oxygen supply device | RUSKINN | INVIV | |
| C57BL/6J mice | Charles River. Germany | no catalog number | |
| Carprofen | Zoetis | Rimadyl 50 mg/ml | |
| CATHETER-FEP 26G | TERUMO | Surflo-W | |
| Clip Applicator Forceps Style | FST | 18057-14 | |
| Curved forceps | WPI | 14114-G | |
| custodiol/HTK | Dr. Franz Köhler Chemie | no catalog numer | |
| Cutasept skin disinfection | VWR | BODL980365 | |
| electrosurgical pen | Bovie | CHANGE-A-TIP | |
| gauze pads, cotton swabs | Lohmann-Rauscher | 13353 | |
| Heating mat | THERMO MAT PRO 30W | HTP-30 | |
| Hemostatic sponge | CuraSpon | J1276A | |
| heparine-solution | Haus-Apotheke | PZN 03029820 | |
| Ice box | PETZ | No Catalog Number available | |
| Inhalation anesthesia device | GROPPLER | BKGM 0616 | |
| insulation and oxygen supply device | RUSKINN | INVIV | |
| isoflurane | CP Pharma | Isofluran CP 1 ml/ml | |
| ketamine | Zoetis | no catalog numer | |
| metamizole | WDT | no catalog numer | |
| Micro scissors | FST | 15000-00,15000-10 | |
| Micro Serrefine ( Clamp ) Angled / 16 mm | FST | 18055-06 | |
| Microscope | Leica | LEICAMZ6 | |
| Microscope light | SCHOTT | KL2500LED | |
| Saline solution (NaCl 0.9%) | Haus-Apotheke | PZN 06178437 | |
| Scissors | Peha Instruments | 991083/4 | |
| small Petri dish | Sarstedt | 833900 | |
| Straight forceps | WPI | 14113-G | |
| surgical tape | BSN | 4120 | |
| Suture Tying Forceps – 10 cm | FST | 18025-10 | |
| Sutures(10-0) | Medtronic | N2540 | |
| Sutures(4-0) | ETHILON | V4940H | |
| Sutures(7-0) | ETHILON | 1647H | |
| Syringe (0.3 mL) | BD | 324826 | |
| Syringe (1 mL) | BD | 320801 | |
| xylazine | Bayer | Rompun |
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Tags
Blood Circuit ReconstructionAbdominal MouseHeart TransplantationAlloimmune ResponseSurgical TechniqueHeterotopic Abdominal Heart TransplantationDonor AortaRecipient Abdominal AortaDonor Inferior Vena CavaRecipient Inferior Vena CavaAnesthesiaExsanguinationHTK SolutionAortic ArchPulmonary ArterySuperior Vena CavaInferior Vena CavaPulmonary Vein
Citazione di questo articolo
Yin, D., Fu, J., Allabauer, I., Witzke, O., Rong, S., Hoerning, A. Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model. J. Vis. Exp. (172), e62007, doi:10.3791/62007 (2021).