快速模型，以评估C57BL6/j小鼠美联储高脂肪饮食中芳烃（Clove）和孜然细胞（Cumin）组合的抗肥胖潜力
Summary
这项工作提出了一个协议，探索两种植物一起使用的抗肥胖效果，为期5周。肥胖小鼠的提取物和高脂肪饮食（HFD）有一个组合管理。这种方法可以促进植物在治疗肥胖症方面的好处。
Abstract
一些研究表明，植物的植物化学成分是潜在的抗肥胖剂。在这项研究中，我们考察了使用来自芳 香酸锡锡的 干按钮和来自 孜然细胞 （CC）的种子对C57BL6/J小鼠通过高脂肪饮食（HFD）诱导肥胖的影响。这项研究的目的是证明，在经过几个星期的实验后，研究中提出的方法显著降低了肥胖症。两种植物的提取物都是用超声波提取的，以增强植物化学物质的提取。乙醇获得最佳提取条件如下：50：50 v/v水，超声波功率为300 W，超声波时间为30分钟。在 HFD 中同时施用 CC 提取物 5 周，可调节脂质轮廓（胆固醇和甘油三酯）、减少食物摄入量、体重增加、脂肪组织和肝脏重量。这个肥胖模型获得的结果表明CC提取物可以预防肥胖。与传统的16周方法（8周发胖，8周减肥）相比，本研究肥胖模型在实验时间较短时也得出了类似的结果。
Introduction
身体脂肪积累过多是肥胖的一个特征。能量摄入和消耗之间的不平衡导致脂肪细胞中储存多余的能量，这与2型糖尿病、高血压、高胆碱血症和心血管疾病1、2中高血糖和胰岛素抵抗的代谢危险因素有关。
天然产品与最小的副作用和低成本已得到越来越多的关注，因为以前的研究已经报告生物活性植物化学物质和潜在的抗肥胖剂的机制，逆转或延迟代谢综合征和相关病理8。
已研究了几种药用植物，以防止肥胖和相关疾病。其中，芳香素在3T3-L1细胞体外治疗和高脂肪饮食11喂养的小鼠体外治疗中具有抗肥胖潜力， 已被调查。此外，在12日对极度肥胖受试者的Cminum cyminum多中心公开试验中观察到显著的抗超重效果。在这项研究中，C57BL6/J小鼠被用来研究实验快速模型，使用两种可食用植物的组合来评估潜在的抗肥胖剂。
Protocol
所有动物实验均得到国家动物实验委员会（IPN） 的批准：第2732号议定书）。 1. 准备丁香和孜然结合的提取物 注：可食用的植物，芳 香素 （丁香）和 孜然（ 孜然）从墨西哥阿巴斯托CDMX中心购买。 从植物中研磨500克种子，进行超声波辅助提取。用乙醇（60：40 v/v）用乙醇（320瓦;24 kHz频率，30分钟）将它们（320瓦;24千赫频率<sup class="x…
Representative Results
超声波辅助提取提取的最佳提取条件是乙醇：水（50：50，v/v），超声波功率为300 W，超声波时间为30分钟。这样，提取速度比传统提取方法（表2）快。提取物显示紫外可见光谱（图1）在320纳米处有强烈的吸收峰值。 食物摄入量和体重在 图2A中显示小鼠体重的变化，这些小鼠的提取物浓度不同（100、…
Discussion
在这项研究中，我们首次评估了用高脂肪饮食喂养5周的小鼠中丁香和孜然（CC）提取物组合的口服对脂质轮廓和肥胖症的影响。结果显示，HFD组的体重比ND组显著增加，这表明肥胖模式的肥胖感应是成功的。施用的CC剂量（100、200和450毫克/公斤/天）可减少食物摄入量和体重。因此，食物摄入量的减少必须是由于动物食欲下降。
减少血脂异常对预防肥胖相关疾病非常重要。在?…
Divulgazioni
The authors have nothing to disclose.
Acknowledgements
这项研究得到了墨西哥国家政治研究所的支持。
Materials
| Distilled Water | Any vendor | n/a | Available for other vendors as well |
| Ethanol | Fermont | 6063 | 99.8% purity |
| Diet Ingredients | |||
| Casein | Any vendor | n/a | |
| Cellulose | Any vendor | n/a | |
| Centrum balance multivitamin | Pfizer | n/a | |
| Choline Bitartrate | Any vendor | n/a | |
| L- cystein | Sigma Aldrich | 168149 | Available for other vendors as well |
| Lard | Any vendor | n/a | |
| Maltodextrin 10 | Any vendor | n/a | |
| Pellets Nutricubos | Purina | n/a | Available for other vendors as well |
| Soybean Oil | Any vendor | n/a | |
| Sucrose | Any vendor | n/a | |
| Extraction Equipment | |||
| Rotavapor | Buchi | R-300 | |
| Shimadzu UV-1800 UV/Visible Scanning Spectrophotometer | Cole Parmer | T-83400-20 | Available for other vendors as well |
| Ultrasonic Unit | Elma | TI-H-20 | Available for other vendors as well |
| Vacuum pump | Buchi | V-100 | |
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Citazione di questo articolo
Pérez Gutiérrez, R. M., Arrioja, M. W. Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet. J. Vis. Exp. (173), e62087, doi:10.3791/62087 (2021).