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使用 zSpRY-ABE8e 对斑马鱼进行人类遗传病建模的高效无 PAM 碱基编辑
JoVE Journal
Biologia
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JoVE Journal Biologia
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
DOI:

07:31 min

February 17, 2023

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Capitoli

  • 00:04Introduction
  • 01:02Preparation of Microinjection Capillaries and Modified RNA
  • 02:23RNA Microinjection of Zebrafish Embryos
  • 04:36Efficiency Analysis of EditR Base Edits
  • 06:08Results: TSR2 Diamond Blackfan Zebrafish Mutation
  • 06:41Conclusion

Summary

Traduzione automatica

该协议描述了如何在没有PAM限制的情况下进行有效的腺嘌呤碱基编辑,以使用zSpRY-ABE8e构建精确的斑马鱼疾病模型。

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