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Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties
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Biologia
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JoVE Journal Biologia
Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

DOI:
10.3791/1032-v

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12:20 min

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November 03, 2008

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, , ,
1Department of Physiology and Biophysics,Weill Cornell Medical College, 2Department of Physiology and Biophysics,Weill Cornell Medical College of Cornell University

Capitoli

  • 00:00Titolo
  • 00:12Introduction
  • 01:18Preparing for Bilayer Experiments
  • 03:44Forming a Membrane
  • 04:43Gramicidin Channels
  • 05:43Recording the Control Current Traces
  • 06:38Recording the Experimental Current Traces
  • 07:41Data Analysis
  • 11:54Conclusion

Summary

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Membrane protein function is regulated by the cell membrane lipid composition. This video-article details how to form a patch using bilayer patch electrodes, as well as how to use gramicidin channels as reporters of altered membrane properties.

Tags

Single Molecule MethodsMonitoringChangesBilayer Elastic PropertiesMembrane Protein FunctionCell Membrane Lipid CompositionLipid-protein InteractionsLipid Bilayer-protein InteractionsPharmacological ResearchPharmaceuticalsLipid Bilayer Material PropertiesAltered Membrane Protein FunctionGramicidin ChannelsConformational ChangesGramicidin SubunitsProperties Of The LipidGramicidin Channel CurrentReporter Compounds

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