This content is Free Access.
Capitoli
- 00:00Titolo
- 00:07Introduction
- 00:50Electrophysiology Setup
- 01:56Chamber Preparation
- 04:18Glass Electrode Preparation
- 07:21Silver Electrode Preparation
- 08:21Pre-Painting
- 09:02Conclusion
Planar lipid bilayers, also called artificial lipid bilayers, allow you to study ion-conducting channels in a well-defined environment. Here, we demonstrate the individual steps needed to prepare the bilayer chamber, the electrodes and how to test that the bilayer is suitable for single-channel measurements.
Tags
Artificial BilayersElectrophysiology ExperimentsPlanar Lipid BilayersIon-conducting ChannelsMembrane-active PeptidesReconstitution Of Ion ChannelsLipid Bilayer PropertiesBilayer-spanning ChannelsPlanar Bilayer FormationIsolating Patches From BilayerGramicidin ChannelsLipid Bilayer Elastic PropertiesBilayer Chamber PreparationElectrodes PreparationSingle-channel Measurements
