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光致交联未修改蛋白（PICUP）适用于淀粉样肽
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Biologia
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JoVE Journal Biologia
Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides
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光致交联未修改蛋白（PICUP）适用于淀粉样肽

DOI:
10.3791/1071-v

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08:40 min

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January 12, 2009

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, ,
1Department of Neurology, David Geffen School of Medicine,University of California, Los Angeles, 2Brain Research Institute, Molecular Biology Institute,University of California, Los Angeles, 3Department of Neurology,University of California, Los Angeles

Capitoli

  • 00:00Titolo
  • 00:53Introduction
  • 01:50Preparing The Peptides
  • 03:32Solubilizing and Photo-Cross-Linking the HFIP-Treated Peptide
  • 06:22Representative Result
  • 07:54Conclusion

Summary

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Traduzione automatica

光致交联未修改的蛋白质（PICUP）允许低聚物在亚稳态的蛋白质混合物的粒度分布的特性。我们展示PICUP应用程序的三个有代表性的淀粉样肽的40 - 42残留的淀粉样β-蛋白和降钙素形式，控制肽生长激素释放因子。

Tags

Photo-Induced Cross-Linking Of Unmodified ProteinsPICUPAmyloidogenic PeptidesProtein Misfolding DiseasesAlzheimer’s DiseaseParkinson’s DiseaseHuntington’s DiseaseHereditary Amyotrophic Lateral SclerosisType 2 DiabetesOligomer Size DistributionClassical MethodsElectrophoresisChromatographyFluorescenceDynamic Light ScatteringMetastable MixturesCovalent Cross-linkingSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)Size-exclusion Chromatography (SEC)VisualizationQuantitative AnalysisAssembly MonitoringSequence ModificationsOligomerization Mechanism

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