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制作生肌工程的组织结构
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Biologia
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JoVE Journal Biologia
Fabrication of Myogenic Engineered Tissue Constructs
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制作生肌工程的组织结构

DOI:
10.3791/1137-v

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13:43 min

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May 01, 2009

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1Department of Anesthesiology,Children’s Hospital Boston and Harvard Medical School, 2Perioperative and Pain Medicine,Children’s Hospital Boston and Harvard Medical School

Capitoli

  • 00:00Titolo
  • 00:47Introduction
  • 01:23Assembly of Construct Casting Molds
  • 03:25Preparation for Myoblast Cell Isolation
  • 04:56Myoblast Isolation from Skeletal Muscle
  • 09:09Casting Engineered Tissue Constructs
  • 13:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们展示了制造胶原蛋白为基础，组织骨骼肌成肌细胞构造。这些工程结构的3 - D可用于更换或修复组织<em>在体内</em>。对于我们而言，我们设计的这些电气管道修复完整的心脏传导阻滞房室<sup“[1]</sup>。

Tags

FabricationElectronic PacemakersPediatric PatientsCardiac Rhythm DisordersCollagen-based MatrixAutologously-derived CellsRecurrent SurgeriesQuality Of LifePrimary Skeletal Myoblast Cell CulturesHydrogel MatrixElectrical ConduitUpper And Lower Chambers Of The HeartAtrioventricular Electrical ConductionComplete Heart BlockMyoblastsSkeletal MusclesNeonatal Lewis RatsAntibioticsType 1 CollagenMatrigel™NaHCO3

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