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Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
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Biologia
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JoVE Journal Biologia
Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells

Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells

DOI:
10.3791/1156-v

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12:24 min

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May 06, 2009

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,
1Department of Pathology,University of Washington

Capitoli

  • 00:05Titolo
  • 01:07Introduction
  • 01:33Preparation of Aging Cultures
  • 03:03Taking a Viability Age-Point
  • 04:43Loading the Bioscreen C MBR Machine
  • 06:26Data Analysis
  • 10:59Representative Results
  • 11:51Conclusion

Summary

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Chronological aging in yeast refers to the loss of cell viability associated with time in stationary phase. Here we describe a high-throughput method for quantitatively determining yeast chronological life span.

Tags

YeastChronological LifespanAging ResearchReplicative AgingHigh-throughput MethodQuantitative MeasurementDefined MediumAgitationConstant TemperatureAging CultureRich Growth MediumGrowth CurveBioscreen C MBR MachineAlgorithmViable CellsReproducibilityPrecisionGenetic ScreensChemical ScreensLongevity Modifiers

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