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Capitoli
- 00:00Introduction
- 00:17Preparation of dishes
- 00:18Dish for oocyte incubation KSOM
- 01:08Dish for oocyte isolation HCZB and HCZB/hyaluronidase
- 02:23Dish for enucleation and nuclear transfer HCZB/cytocholazin
- 03:30Preparation of pipettes
- 03:31Holding pipette
- 04:53Injection pipette
- 06:30Mouth pipette for oocyte isolation
- 07:07Oocyte isolation
- 09:43Nuclear transfer
- 09:58Enucleation
- 10:31Collection of donor cells
- 11:05Transfer
- 11:50Discussion
