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测量中使用FM标签的神经元的胞外分泌
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Biologia
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JoVE Journal Biologia
Measuring Exocytosis in Neurons Using FM Labeling
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测量中使用FM标签的神经元的胞外分泌

DOI:
10.3791/117-v

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06:56 min

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November 30, 2006

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,
1Department of Molecular and Cell Biology,Harvard

Capitoli

  • 00:04Introduction
  • 00:18Preparation of solutions
  • 00:54Assembling imaging system
  • 02:26Perfusion
  • 03:20Imaging
  • 04:47Discussion

Summary

Traduzione automatica

Traduzione automatica

囊泡释放动力学的能力来衡量，可以帮助提供洞察到一些神经递质的基础。在这里，我们采用实时成像与红色荧光染料FM 4-64标记，以测量在海马神经元的文化突触前囊泡释放率的囊泡。

Tags

MeasuringExocytosisNeuronsFM LabelingKineticsVesicle ReleaseReal-time ImagingFM DyePresynapticEndocytosisFluorescenceSynaptic VesiclesLipophilic InteractionsBright SignalExtracellular FluidWash StepPlasma MembraneExpelledElectrical StimulationRate Of ReleaseFluorescence DecreaseNeuronal CulturesTransfected SynapsesControl Boutons

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