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Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay
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Biologia
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JoVE Journal Biologia
Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay

Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay

DOI:
10.3791/1191-v

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19:34 min

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April 03, 2009

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, , , ,
1Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy,Tabriz University (Medical Sciences), 2Gifted and Talented Students Office, Educational Development Center,Tabriz University (Medical Sciences), 3School of Advanced Biomedical Sciences,Tabriz University (Medical Sciences)

Capitoli

  • 00:00Introduction
  • 00:15Experiment
  • 17:48Conclusion

Summary

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The MTT assay is an easy and reproducible colorimetric assay for evaluation of cell viability based on reduction of yellow MTT and production of water insoluble purple formazan. Here, the viability of MCF-7 cells upon treatment of nanogenomedicine has been evaluated.

Tags

Cellular ToxicityNanogenomedicineMCF-7 Cell LineMTT AssayCytotoxicityShort Interfering RNAAntisenseGene-based MedicineCarrierColorimetric ApproachMitochondrial Dehydrogenase EnzymesNanopolyplexesWater Soluble Yellow MTTFormazanDimethylsulfoxideSornson’s Buffer PH 10.5Spectrophotometry

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