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Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (Danio rerio)
JoVE Journal
Biologia
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JoVE Journal Biologia
Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (Danio rerio)
DOI:

08:03 min

May 08, 2009

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Capitoli

  • 00:00Titolo
  • 00:55Introduction
  • 01:33Preparation of the Tissue
  • 03:06Gross Dissection of the Inner Ear
  • 04:01Fine Dissection of the Inner Ear Sensory Epithelia
  • 06:00Fluorescent Staining of the Hair Cells in the Sensory Epithelia
  • 06:53Representative Results/ Outcome
  • 07:31Conclusion

Summary

Traduzione automatica

The inner ear sensory epithelium of adult zebrafish is a good model system for understanding the mechanisms of hair cell regeneration in adult vertebrates. This protocol demonstrates the fine dissection of the epithelia, through which we can get tissue samples for studying the regenerative events at cellular and subcellular levels.

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