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Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies
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Biologia
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JoVE Journal Biologia
Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies

Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies

DOI:
10.3791/1304-v

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12:03 min

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September 30, 2009

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,
1Department of Veterinary Integrative Biosciences,Texas A&M University (TAMU)

Capitoli

  • 00:00Titolo
  • 00:52Introduction
  • 01:27Preparing for Dissection
  • 02:02Harvesting the E16 Rat Embryos
  • 03:19Isolating the Forebrains
  • 04:25Digesting and Triturating the Forebrain Tissue
  • 06:09Washing, Filtering, and Counting the Dissociated Cells
  • 07:03Culturing the Dissociated Cells to Promote Aggregation
  • 07:54Preparing the Coverslips and Washing the Aggregates
  • 09:15Seeding the Coverslips with the Aggregates
  • 11:06Representative Connections Formed Among Aggregates
  • 11:29Conclusion

Summary

Traduzione automatica

Traduzione automatica

A protocols for an embryonic rat brain aggregate culture system is described. Multipotent progenitors in the aggregates can develop and differentiate into neurons, astrocytes and oligodendrocytes.

Tags

Rat Brain Aggregate CulturesNeuron Development StudiesGlia Development StudiesIn Vitro SystemCentral Nervous System (CNS)ProgenitorsMature NeuronsGliaAxo-glial InteractionsMultipotent ProgenitorsOligodendroglial Lineage CellsCellular And Molecular LevelSerum-free MediumEmbryonic Rat ForebrainsNeuron-glia InteractionCNS MyelinationIsolate And Grow CNS Aggregate CulturesE16 Rat BrainRegular Dissociated Neuronal Cultures

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