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Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
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JoVE Journal Biologia
Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

DOI:
10.3791/1305-v

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11:54 min

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June 09, 2009

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,
1Cellular and Molecular Physiology,Yale University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:00Introduction
  • 01:29Dissection and Bipolar Cell Isolation
  • 05:11Bipolar Cell Loading and Wash Out
  • 08:26Patch Clamping and TIRFM Imaging
  • 10:38Representative TIRFM Imaging Results
  • 11:23Conclusion

Summary

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Traduzione automatica

In this video, we demonstrate how to label and visualize single synaptic vesicle exocytosis and trafficking in goldfish retinal bipolar cells using total internal reflectance fluorescence (TIRF) microscopy.

Tags

TIRF MicroscopyExocytosisRetinal Bipolar CellsSynaptic VesiclesGoldfish RetinaPatch ClampingPre-synaptic VoltageSynaptic ReleaseFluorescent DyeFM 1-43®DepolarizesEndocytosisGlutamatergic VesiclesPatch Pipette SolutionRhodamine-based PeptideSynaptic Ribbon Protein RIBEYEActive Zones

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