/

/

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

JoVE Journal
Biologia

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

DOI:

•

19:16 min

•

August 05, 2009

•

James Lim, Gaudenz Danuser

¹Department of Cell Biology,Scripps Institute

Capitoli

00:05Titolo
00:58Introduction
01:24Preparing Fluorescently Labeled Actin and Cells for Microinjection
05:46Microinjection of Labeled Actin
10:14Assembling the Imaging Chamber
15:20Imaging Cells
18:17Representative Results: Microinjected Cells
18:44Conclusion

Summary

Traduzione automatica

Selection, microinjection, and imaging of fluorescently-labeled F-actin via fluorescent speckle microscopy (FSM).

Tags

Fluorescent Speckle Microscopy F-actin Dynamics Live Cell Imaging FSM Actin Dynamics PtK1 Cells Turnover Kinetics Assembly/disassembly Computer Vision Software QFSM Fluorescent Actin Probes Macromolecular Assemblies Microtubules Intermediate Filaments Adhesion Complexes

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Read Article