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Live cell imaging van de F-actine Dynamics via Fluorescent Speckle Microscopy (FSM)

JoVE Journal
Biologia

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JoVE Journal Biologia

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

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Live cell imaging van de F-actine Dynamics via Fluorescent Speckle Microscopy (FSM)

DOI:

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19:16 min

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August 05, 2009

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James Lim, Gaudenz Danuser

¹Department of Cell Biology,Scripps Institute

Capitoli

00:05Titolo
00:58Introduction
01:24Preparing Fluorescently Labeled Actin and Cells for Microinjection
05:46Microinjection of Labeled Actin
10:14Assembling the Imaging Chamber
15:20Imaging Cells
18:17Representative Results: Microinjected Cells
18:44Conclusion

Summary

Traduzione automatica

Selectie, micro-injectie, en de beeldvorming van fluorescent-gelabeld F-actine via fluorescentie microscopie spikkel (FSM).

Tags

Fluorescent Speckle Microscopy F-actin Dynamics Live Cell Imaging FSM Actin Dynamics PtK1 Cells Turnover Kinetics Assembly/disassembly Computer Vision Software QFSM Fluorescent Actin Probes Macromolecular Assemblies Microtubules Intermediate Filaments Adhesion Complexes

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