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Generation of Human CD40-activated B cells
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Biologia
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JoVE Journal Biologia
Generation of Human CD40-activated B cells

Generation of Human CD40-activated B cells

DOI:
10.3791/1373-v

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13:27 min

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October 16, 2009

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, , , ,
1Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program,University Hospital of Cologne, Department I of Internal Medicine

Capitoli

  • 00:00Titolo
  • 00:14Introduction
  • 01:46Preparation of Feeder Cells
  • 02:27Preparation of PBMC for CD40- Stimulation
  • 04:38Preparation of Feeder Cells
  • 08:33CD40- Stimulation
  • 09:50Subcultivation
  • 12:20Analysis of CD40- Activated B Cells
  • 12:55Conclusion

Summary

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In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.

Tags

CD40-activated B CellsCancer ImmunotherapyAntigen-presenting CellsMHCCo-stimulatory MoleculesInterleukin-4CD40 LigandLymph Node Homing TriadPeripheral BloodIn Vitro ExpansionCell CultureCyclosporin ARecombinant Human CD40 Ligand

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