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小动物的肌肉植入动态跟踪在体内荧光成像
JoVE Journal
Biologia
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JoVE Journal Biologia
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
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小动物的肌肉植入动态跟踪在体内荧光成像

DOI:
10.3791/1388-v

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08:15 min

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September 21, 2009

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, , , ,
1Department of Anesthesia,Brigham and Woman’s Hospital, 2Department of Radiology,Brigham and Woman’s Hospital

Capitoli

  • 00:00Titolo
  • 00:11Introduction
  • 00:36Preparing GFP-Labeled Cells
  • 03:40Transplanting Cells and in vivo Fluorescence Imaging
  • 06:29In vivo Fluorescence Imaging Results
  • 07:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述了一个<em>在体内</em>荧光成像协议监测GFP标记成肌细胞后，移植到健康和营养不良小鼠的骨骼肌肌肉再生。该协议可以适应其他肌肉条件，以及研究其他类型的细胞移植和肌肉再生。

Tags

Muscle EngraftmentSmall AnimalsIn Vivo Fluorescent ImagingMuscular DystrophiesDegenerative Muscle DiseasesStem Cell BiologyCell-based TherapiesFluorescent ProteinsTransplantationNon-invasive TechniqueMuscle Engraftment EvaluationIn Vivo Fluorescence ImagingGFP-labeled CellsMouse Skeletal MusclesBackground ConcernHair Removal ReagentsCCD CameraTibialis Anterior MuscleExtensor Digitorum Longus Muscle

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