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一个外源凝集素的高效液相色谱法从复杂生物样品，以丰富的选择性糖基化肽

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

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一个外源凝集素的高效液相色谱法从复杂生物样品，以丰富的选择性糖基化肽

DOI:

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20:23 min

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October 01, 2009

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Eric Johansen, Birgit Schilling, Michael Lerch, Richard K. Niles, Haichuan Liu, Bensheng Li, Simon Allen, Steven C. Hall, H. Ewa Witkowska, Fred E. Regnier, Bradford W. Gibson, Susan J. Fisher, Penelope M. Drake

¹Obstetrics, Gynecology and Reproductive Sciences,University of California, San Francisco – UCSF, ²Buck Institute for Age Research, ³Department of Chemistry,Purdue University

Capitoli

00:00Titolo
00:55Introduction
01:42Preparing Lectin-Conjugated POROS Columns
05:15Packing the Lectin-POROS Beads into a PEEK Column
08:11HPLC of Samples
11:49Desalting the Collected Fractions and PNGase F-digestion
14:50Ziptip Desalting of PNGaseF Samples
16:34Glycopeptide Analysis Representative Results
19:45Conclusion

Summary

Traduzione automatica

凝集素结合POROS珠被雇用的高效液相色谱法。糖肽类标准担任阳性和阴性对照。 MARS的14枯竭，胰蛋白酶消化人体血浆层析和流过（FT）和约束分数ESI-LC-MS/MS分析收集。糖肽的富集在绑定的分数相比，FT。

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