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Capitoli
- 00:00Titolo
- 00:10Introduction
- 01:50Preparation and Dissection
- 06:33Mounting Hearts from Adult Flies
- 08:15Mounting Larval Hearts
- 09:35Fluorescent Labeling of Drosophila Heart Structures: Results
- 10:33Conclusion
Here we describe a basic protocol for fluorescent labeling of different elements of heart tubes from larva and adult Drosophila melanogaster. These specimens are well-suited for imaging via fluorescent or confocal microscopy. This technique permits detailed structural analysis of the features of the hearts from a powerful model organism.
Tags
Fluorescent LabelingDrosophila Heart StructuresDrosophila MelanogasterDorsal VesselCardiomyocytesPericardial CellsAlary MusclesVentral Longitudinal Muscle CellsMyofibrilsCytoskeletal/submembranous Protein ComplexesExtracellular MatrixFixationFluorescent Labeling ProtocolSarcomeric F-actinα-actininMyosin-GFP Expressing Adult FliesPericardinType IV Extracellular Matrix CollagenMounting StrategySemi-intact Adult HeartsImagingConfocal Microscopy
