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人死后脑组织中的单细胞群获得高品质的RNA
JoVE Journal
Biologia
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JoVE Journal Biologia
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
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人死后脑组织中的单细胞群获得高品质的RNA

DOI:
10.3791/1444-v

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18:17 min

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August 06, 2009

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, ,
1Department of Structural and Molecular Neuroscience,McLean Hospital, 2Department of Psychiatry,Harvard Medical School, 3Department of Psychiatry,Beth Israel Deaconess Medical Center

Capitoli

  • 00:05Titolo
  • 01:06Introduction
  • 01:43Sectioning of Tissue and Staining of Pyramidal Neurons
  • 05:38Single Cell Laser-Capture Microdissection (LCM)
  • 10:48Isolating RNA
  • 13:39Representative Results
  • 17:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述一个过程，利用激光捕获显微切割分离和提取RNA从一个同质的细胞群，锥体神经元，在上级死后人类大脑颞上回第三层。其后，我们线性放大（T7基于）的mRNA，Affymetrix公司人类X3P芯片杂交样品。

Tags

High Quality RNASingle Cell PopulationsGray Matter ReductionSuperior Temporal GyrusSchizophrenia PatientsGene Expression ProfilesPyramidal NeuronsCerebral CortexLaser-capture Microdissection (LCM)Histogene Staining SolutionArcturus XT LCM SystemT7-based Linear AmplificationExperion LabChip (Bio-Rad) GelElectropherogramMRNATranscript LengthMicroarraysHuman X3P GeneChip Probe ArrayAffymetrix

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