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变性尿素聚丙烯酰胺凝胶电泳（尿素页）
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Biologia
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JoVE Journal Biologia
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
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变性尿素聚丙烯酰胺凝胶电泳（尿素页）

DOI:
10.3791/1485-v

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08:44 min

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October 29, 2009

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, ,
1School of Biological Sciences,Nanyang Technological University, Singapore – NTU, 2Singapore-MIT Alliance for Reserach and Technology (SMART)

Capitoli

  • 00:00Titolo
  • 00:08Introduction
  • 00:52Gel Preparation
  • 02:37Prerun
  • 04:03Load Samples and Run the Gel
  • 05:43Process the gel

Summary

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变性尿素聚丙烯酰胺凝胶电泳用于分离单链DNA或RNA的500个核苷酸的限制。在热变性样品和非结构化的单链结合的尿素凝胶基质内迁移，根据其分子量。

Tags

Denaturing Urea Polyacrylamide Gel ElectrophoresisUrea PAGEDNA SeparationRNA SeparationPolyacrylamide Gel MatrixMolecular Weight SeparationAcrylamide ConcentrationLower Molecular Weight FragmentsUnstructured DNA Or RNA MoleculesSingle Stranded DNASingle Stranded RNAOligonucleotidesEnzymatic Cleavage ReactionsGel PreparationGel Running

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