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Depletion of Specific Cell Populations by Complement Depletion
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Depletion of Specific Cell Populations by Complement Depletion

Depletion of Specific Cell Populations by Complement Depletion

DOI:
10.3791/1487-v

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06:17 min

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February 05, 2010

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1BloodCenter of Wisconsin,Blood Research Institute

Capitoli

  • 00:00Titolo
  • 00:45Introduction
  • 01:18Preparation of Rabbit Complement and Antibody Selection
  • 02:22Complement Depletion
  • 05:06Results of the Complement Depletion
  • 05:46Conclusion

Summary

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To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.

Tags

DepletionSpecific Cell PopulationsComplement DepletionImmune Cell PurificationReal-time PCRMicroarray AnalysisFluorescent Activated Cell Sorting (FACS)Magnetic Bead SeparationComplement SystemPlasma ProteinsProteolytic CascadeMembrane-attack ComplexCell DeathClassical PathwayIgM AntibodiesIgG AntibodiesMonoclonal Antibodies (mAb)Antigen-specific MannerRabbit ComplementIncubationWashing

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