È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:54Introduction
- 01:34Preparation of Drosophila
- 04:13Mounting Drosophila Pupae for Imaging
- 05:16Scanning a Tissue of Interest
- 06:18Time-lapse Imaging
- 07:58Three Dimensional Reconstruction of Fluorescence
- 09:59Representative Results/ Outcome
- 10:58Conclusion
Mesoskopisk fluorescens tomografi verkar bortom penetration gränserna för vävnads-snittning fluorescensmikroskopi. Tekniken bygger på multi-projektion belysning och en foton transporter beskrivning. Vi visar in-vivo hela kroppen 3D-visualisering av morfogenes av GFP-uttryckande wing imaginal skivor i<em> Drosophila melanogaster.</em
Tags
Mesoscopic Fluorescence TomographyIn-vivo ImagingDeveloping DrosophilaOptical InterrogationMolecular ChangesFunctional ChangesOptical Imaging MethodsPenetration LimitsOptical MicroscopyDiffusion-imposed LimitsOptical MacroscopyModel OrganismsInsectsAnimal EmbryosSmall Animal ExtremitiesNanometer-resolution Optical ImagingImaging Penetration DepthPhoton ScatteringEntire Embryos ImagingChemical TreatmentScattering ClearancePost-mortem ImagingTwo-photon MicroscopyConfocal MicroscopyBiologia dello sviluppoDrug Discovery
