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A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
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Biologia
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JoVE Journal Biologia
A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

DOI:
10.3791/1513-v

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08:43 min

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August 21, 2009

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1Department of Biology,Concordia University

Capitoli

  • 00:05Titolo
  • 00:49Introduction
  • 01:09Lipid Extraction
  • 05:26Analysis of Extracted Lipids
  • 07:48Analysis of Lipids by Mass Spectrometry Results
  • 08:05Conclusion

Summary

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We describe a new quantitative lipidomics method for identifying numerous lipid species in yeast using survey-scan electrospray ionization mass spectrometry (ESI/MS). This method exceeds currently available methods for lipid identification and quantification in the ability to resolve various molecular forms of lipids, sensitivity, and speed.

Tags

Quantitative AssessmentYeast LipidomeElectrospray Ionization Mass SpectrometryBiomoleculesMembrane DynamicsEnergy StorageSignallingSaccharomyces CerevisiaeLipid SpeciesLipids ClassesChain LengthsDegree Of UnsaturationLipid IdentificationLipid QuantificationHigh Performance Liquid ChromatographyThin-layer ChromatographyFluorescence MicroscopyGas ChromatographyMS AnalysisSurvey-scan ESI/MSLipid MixturesData Acquisition

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