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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Biologia
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JoVE Journal Biologia
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

DOI:
10.3791/1514-v

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09:00 min

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August 04, 2009

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1Sigma Aldrich

Capitoli

  • 00:05Titolo
  • 00:59Introduction
  • 01:21The Protease Assay
  • 06:41Fluorescence Measurement
  • 07:43Fluorescence Measurement of Protease Activity Representative Results
  • 08:29Conclusion

Summary

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The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.

Tags

ProteaseFluorescent Detection KitProtease ActivityAssayCasein SubstrateFluorescein Isothiocyanate (FITC)CleavageAcidificationTrichloroacetic Acid (TCA)CentrifugationPelletAcid Soluble FragmentsSupernatantNeutralizationFluorescence MeasurementTrypsin Protease ControlConcentrationIncubation TimeMicrocentrifuge TubesCuvettesMultiwell Plates

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