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大鼠晶状体上皮细胞外植体的制备及文化学习终末分化
JoVE Journal
Biologia
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JoVE Journal Biologia
Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
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大鼠晶状体上皮细胞外植体的制备及文化学习终末分化

DOI:
10.3791/1519-v

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15:02 min

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September 22, 2009

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, ,
1Laboratory of Molecular and Developmental Biology,National Eye Institute (NEI), National Institutes of Health (NIH)

Capitoli

  • 00:00Titolo
  • 00:54Introduction
  • 02:09Microdissection of Explants
  • 07:16Microdissection and Culture of Central Explants
  • 09:18Harvesting the Explants for Protein Analysis
  • 10:19Harvesting the Explants for Immunofluorescence
  • 12:15Immunofluorescence and Protein Analysis Results
  • 15:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

在FGF - 2的存在培养的大鼠晶状体上皮细胞的中部地区的外植体分化同步。这种文化免疫荧光显微镜，可以提供有关新的信息与终末分化相关的基因表达和信号事件。

Tags

PreparationCultureRat Lens Epithelial ExplantsTerminal DifferentiationAnterior SurfaceOcular LensEpithelial CellsProliferationAnnular ZoneCiliary BodyMigrationPosterior ArrayLens Fiber CellsBulk Of The LensIn Vitro InductionFGF-2Neonatal RatsLens CapsuleDissecting TweezersPeripheral RegionsCentral AreaFGF-2 ConcentrationTime CourseSignalingGene ExpressionMolecular TechniquesBiochemical TechniquesPharmacological TechniquesImmunofluorescence Microscopy

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