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Capitoli
- 00:00Titolo
- 01:16Introduction
- 01:49Plasmid Preparation for Electroporation
- 02:46Animal Preparation for Electroporation
- 06:12Electroporation and Transfection
- 08:31Assaying In-Vivo Transfection by Electroporation
- 08:36Transfection Efficiency
- 09:47Subcellular Protein Localization
- 12:31Expression and Targeting of the a-sub-unit of the Dihydropyridine Receptor (DHPR-a1s)
- 13:56Functional Evaluation of the Expression of EYFP-CIC1
- 15:30Conclusion
Nous décrivons des procédures détaillées pour la transfection efficace de l'ADN plasmidique dans les fibres des muscles du pied des souris vivantes en utilisant l'électroporation et la visualisation ultérieure de l'expression des protéines en utilisant la microscopie à fluorescence.
Tags
DNA TransfectionMammalian Skeletal MusclesIn Vivo ElectroporationCell BiologyTransgenic ProteinsFluorescently Tagged ConstructsMutant VariantsIn Vivo TransfectionPlasmidsLiverSkeletal MuscleBrainFlexor Digitorum BrevisInterosseus MusclesAdult MiceExperimental ParametersSoluble ProteinsStructural ProteinsMembrane ProteinsEGFPECFP3CalpainFKBP12β2a-DHPRMinidystrophinα-actininα1s-DHPRRyR1Cardiac Na/Ca2+ ExchangerNaV1.4 Na ChannelSERCA1
