Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Rivista

Biologia

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

DOI:

10.3791/1529-v

•

15:57 min

•

October 09, 2009

•

Nathan A. Tanner, Joseph J. Loparo, Antoine M. van Oijen

¹Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Capitoli

00:00Titolo
00:42Introduction
01:11Preparing the DNA Replication Template
02:44Preparing Functionalized Coverslips
05:46Assembling the Flow Chamber
10:03Single-Molecule Replication Experiment
13:18Single Molecule DNA Replication Data Analysis and Representative Results
15:00Conclusion

Summary

Traduzione automatica

This protocol demonstrates a simple single-molecule fluorescence microscopy technique for visualizing DNA replication by individual replisomes in real time.

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

•

•

•

Capitoli

Summary

Traduzione automatica

Tags

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Time-lapse Imaging of Mitosis After siRNA Transfection

Cell Electrofusion Visualized with Fluorescence Microscopy

Single-Molecule Imaging of Nuclear Transport

Overvåking plasmidreplikasjon i Live pattedyrceller over flere generasjoner av fluorescensmikropskopi

Kombinere Single-molekyl Manipulasjon og bildebehandling for studier av protein-DNA interaksjoner

Studerer DNA Looping av Single-Molecule FRET

Visualisering Stromule Frequency med Fluorescensmikroskopi

Read Article