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特定细胞群的净化荧光激活细胞分选（FACS）
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
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特定细胞群的净化荧光激活细胞分选（FACS）

DOI:
10.3791/1546-v

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15:29 min

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July 10, 2010

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, , ,
1Blood Research Institute,BloodCenter of Wisconsin

Capitoli

  • 00:05Titolo
  • 00:08Introduction
  • 00:38Materials and Preparation for FACS
  • 02:17Cell Staining & Prep of Control Samples
  • 07:11Flow Cytometer Setup
  • 08:56Flow Cytometer Compensation
  • 11:58Experimental Sample Gating & Sorting
  • 14:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

许多科学研究需要的细胞群的生物和化学分析，细胞必须在高纯度的状态。荧光激活细胞分选（FACS）是一种高级的方法，在其中获得纯的细胞群。

Tags

PurificationSpecific Cell PopulationFluorescence Activated Cell SortingFACSTechniquePhenotypeBulk MethodsPanningComplement DepletionMagnetic Bead SeparationAdvantagesHigh PurityIdentifying MarkerDifferential Marker DensityInternal StainingIntracellular Protein ExpressionGenetically Modified Fluorescent Protein MarkerSizeGranularityFluorescenceFluorescently-tagged Monoclonal AntibodiesNegative SelectionFlow CytometerSorting CapacitySoftware

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