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Capitoli
- 00:00Titolo
- 00:57Introduction
- 01:29Transfection of Plasmids for Production of Renilla-antigen Fusion Proteins
- 03:28Harvesting Renilla-antigen Fusions
- 05:12Preparing a Sera Master Plate
- 06:30LIPS Assay
- 09:01Measuring Luminescence and Data Analysis
- 10:49LIPS Assay as a Method for Rapid and High Thoughput Sera Antigen Analysis
- 11:25Conclusion
LIPS (Lusiferaz Immunoprecipitation Sistemleri) yerine teknik yönleri anlatılmaktadır. Genel yaklaşım, memeli hücrelerinde Renilla lusiferaz (Ruc) erimiş antijenleri kodlayan kimerik genler ifade içerir. Ham Ruc antijen ekstreleri hazırlanmış ve daha sonra, arıtma olmadan, antikorlar ölçmek için immunoprecipitation deneyleri istihdam.
Tags
Antibody ProfilingLuciferase Immunoprecipitation Systems (LIPS)Comprehensively UnderstandingQuantifying Antibody ProfilesAutoantigensInfectious AgentsDisease MechanismsMarkersSubstratify DiseaseClinical FeaturesPathogenesisHighly Quantitative MethodPatient Sera Antibody ResponsesPathogen AntigensInfectionELISAsHumoral Serological Response ProfilesUniversal FormatDynamic Antibody Titer RangesProteomeHIVInformativeSingle Antigen ELISAPurified AntigensAssay Optimization Steps
