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Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

DOI:

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20:38 min

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February 25, 2007

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Cristina Lo Celso, David Scadden

¹Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute,Harvard Medical School

Capitoli

00:00Introduction
00:20Isolating bone marrow cells
00:21Dissecting mouse to isolate bones
01:03Isolating tibia
01:40Isolating femur
02:12Isolating hip bone
02:50Isolating spine
04:07Cleaning bones
06:37Crushing bones
09:33Lineage depletion of bone marrow cells
14:06Antibody staining for FACS
16:06Transplantation into irradiated mouse
18:50Discussion

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Isolation Transplantation Hematopoietic Stem Cells HSCs

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