È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:00Introduction
- 00:48Dissection of mouse female organs
- 02:21Dissection of uterus under microscope
- 03:51Isolation of embryo
- 06:59Discussion
Isolation of postimplantation-stage embryos allows one to study gene patterning and analyze cell-lineage decision making processes during embryonic development, but proper dissection of the early embryo can be challenging. This protocol describes a method for isolating early primitive-streak-stage embryos (~6.5 days post coitum [dpc]).
Tags
Dissection6.5 DpcMouse EmbryosGene Expression PatternsEarly StagesMammalian Embryonic DevelopmentGene FunctionCell-cell InteractionSignaling MechanismsEmbryonic PatterningDeciduaImplantationStep-by-step DocumentationPost-implantation EmbryosUterusPregnant MouseVaginal PlugMaternal DeciduaReichert’s MembraneEctoplacental Cone
