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Digital Microfluidics for Automated Proteomic Processing
JoVE Journal
Biologia
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JoVE Journal Biologia
Digital Microfluidics for Automated Proteomic Processing
DOI:

10:55 min

November 06, 2009

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Capitoli

  • 00:00Titolo
  • 00:55Introduction
  • 01:29Microfluidic Device Fabrication
  • 04:53Microfluidic Device Set-up
  • 06:13Sample and Reagent Preparation
  • 07:22Digital Microfluidic Sample Processing
  • 09:15Post-Processing Sample Preparation
  • 09:52Protein Purification by DMF
  • 10:17Conclusion

Summary

Traduzione automatica

Digital Microfluidics is a technique characterized by the manipulation of discrete droplets (~nL - mL) on an array of electrodes by the application of electrical fields. It is well-suited for carrying out rapid, sequential, miniaturized automated biochemical assays. Here, we report a platform capable of automating several proteomic processing steps.

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