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游离成年哺乳动物元DNA的细胞核内内微量注射
JoVE Journal
Biologia
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JoVE Journal Biologia
Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
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游离成年哺乳动物元DNA的细胞核内内微量注射

DOI:
10.3791/1614-v

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13:39 min

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December 10, 2009

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, , ,
1Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism (NIAAA),National Institutes of Health (NIH)

Capitoli

  • 00:00Titolo
  • 00:55Introduction
  • 01:39Preparation for Injection and Fabrication of Injection Pipettes
  • 04:54Intranuclear Microinjection
  • 12:00Whole-cell Recordings of Currents through Heterologously-Expressed G-protein Coupled Inwardly Rectifying K+ (GIRK) Channels from SCG Neurons
  • 12:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

核内基因直接注射是有效的有丝分裂后细胞转染技术。此方法提供从单个或多个基因结构的外源蛋白的表达水平高，使蛋白质的功能与生理有关环境的各种单细胞分析研究。

Tags

Intranuclear MicroinjectionDNADissociated Adult Mammalian NeuronsProtein FunctionPrimary Neuronal Cell CulturesHeterologous Protein ExpressionElectroporationChemically-mediated TransfectionCDNA ConstructsAdult Sympathetic NeuronsInverted MicroscopeGlass Injection PipetN2(g) Pressure Delivery SystemMicromanipulatorTransfection MethodsSingle Cell StudiesElectrophysiology

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