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在豚鼠肠道网络，利用电压敏感染料光学记录的电活动

JoVE Journal
Biologia

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JoVE Journal Biologia

Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes

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在豚鼠肠道网络，利用电压敏感染料光学记录的电活动

DOI:

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14:23 min

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December 04, 2009

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Ana L. Obaid, B. M. Salzberg²

¹Department of Neuroscience,University of Pennsylvania-School of Medicine, ²Department of Physiology,University of Pennsylvania-School of Medicine

Capitoli

00:00Titolo
00:59Introduction
01:46Tissue Preparation
05:39Preparation of Tissue Samples for Optical Recording
07:10Optical Recording of Electrical Activity
10:30Image Analysis
12:00Representative Optical Data
13:14Conclusion

Summary

Traduzione automatica

该协议说明了电压敏感染料如何使豚鼠肠神经系统的神经丛，如完整的神经网络电活动的可调节分辨率的光学记录，范围从单细胞到多节后电路。

Tags

Optical Recording Electrical Activity Guinea-pig Enteric Networks Voltage-sensitive Dyes Enteric Nervous System Myenteric Plexus Submucous Plexus Reflex Pathways Ganglia Synaptic Interactions Gastrointestinal Function Experimental Model Molecular Events Cellular Events Neuronal Network Physiological Outputs

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