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Capitoli
- 00:06Introduction
- 01:49Preliminary Steps
- 02:28Loading the cells with di-8-ANEPPS
- 03:21Experiment and image acquisition
- 04:35Image processing and analysis
Campo eléctrico externo provoca una tensión en la membrana de una célula, denominada tensión de la membrana inducida (ΔΦ). Mediante el uso de la tintura potenciométrica di-8-ANEPPS, es posible medir el ΔΦ no invasiva. Este video muestra el protocolo para la medición de ΔΦ con di-8-ANEPPS.
Tags
Induced Membrane VoltageDi-8-ANEPPSCellElectric FieldCharge RedistributionCell MembraneVoltageInduced Transmembrane VoltageInduced Transmembrane Potential DifferenceDelta PhiResting VoltagePotentiometric Fluorescent DyesVariationsNoninvasivelyLipid BilayerFluorescence IntensityProtocolCell ShapeAmplitudeSpatial Distribution
