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植物原生质体的荧光激活细胞分选
JoVE Journal
Biologia
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JoVE Journal Biologia
Fluorescence Activated Cell Sorting of Plant Protoplasts
DOI:

13:35 min

February 18, 2010

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Capitoli

  • 00:00Titolo
  • 00:58Introduction
  • 01:23Preparation of the Plant Material
  • 02:43Harvesting and Protoplasting of the Plant Material
  • 06:10Fluorescence Activated Cell Sorting of Protoplasts
  • 11:26FACS Followed by RNA Extraction of Plant Protoplasts Representative Results
  • 12:59Conclusion

Summary

Traduzione automatica

一个隔离特定的细胞类型,从植物材料的方法是证明。这种技术采用特定类型的细胞,细胞分离和荧光激活细胞分选表达荧光蛋白的转基因标记线。此外,增长的设置是在这里建立,有利于治疗<em>拟南芥</em>细胞分选前的幼苗。

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