Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Primary Culture and Plasmid Electroporation of…
JoVE Journal
Biology
Author Produced

This Contenuto is Open Access.

JoVE Journal Biology
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
 
Click here for the English version

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

Article DOI: 10.3791/1685-v • 12:30 min • February 4th, 2010
February 4th, 2010 •
1,2,3, 1,2, 1,2,4
1Department of Otology and Laryngology, Harvard Medical School, 2Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders, Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology, Harvard

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.

Tags

Primary Culture Plasmid Electroporation Murine Organ Of Corti Sensory Epithelium Audition Cochlea Inner Ear Hair Cells Mechanosensory Cells Inner Hair Cells Outer Hair Cells Basilar Membrane Sensorineural Hearing Loss Auditory Research Organ Explant Gene Expression Stereocilia Motility Cell And Molecular Biology Hair Cell Regeneration Isolation And Culture Method Neonatal Mice Temporal Bone Isolation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter